An overview of where next-generation sequencing is used, from whole-genome variant discovery to clinical liquid biopsy and single-cell transcriptomics.

Understand the stages of a standard RNA-seq bioinformatics workflow, including the role of QC, alignment, gene expression quantification, and specialized application analyses Identify key QC metrics ...

Rapid precision run-on sequencing (rPRO-seq) may help doctors understand disease states and treatment response in real time. Researchers at Sylvester Comprehensive Cancer Center (FL, USA) have ...

The high complexity and diversity of the eukaryotic transcriptome poses significant challenges for the efficient detection of specific transcripts. Conventional targeted RNA-seq methods often require ...

Although there are striking differences between the cells that make up your eyes, kidneys, brain and toes, the DNA blueprint for these cells is essentially the same. Where do those differences come ...

ALBANY, N.Y. (July 6, 2026) — RNA and DNA are molecular cousins. Both carry biological instructions and both are built from four basic building blocks. They also have their differences. One of these ...

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This eBook explores advances in RNA sequencing (RNA-seq), with key content from BioTechniques’ digital hub and Taylor & Francis journals, Biotechnology and Genetic Engineering Reviews, Immunological ...

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

Short-read next-generation sequencing (NGS) transformed genomics by delivering billions of accurate reads at low cost, but a fundamental constraint remains: a read shorter than the feature being ...